Part:BBa_K1697000:Design

SboA from B.subtilis 168

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The operon for SboA is not included

Source

The sequence originally came from the genome of Bacillus subtilis 168, amplified, then inserted into a vector by Gateway reaction. Then the iGEM prefix and suffix was added using PCR. From there, the sequence that was already added with prefix and suffix was cloned into pSB1C3.